Abstract
背景与目的端粒酶逆转录酶为端粒酶的催化亚单位,其活性与细胞对化疗药物的敏感性密切相关。本实验研究人肺腺癌Anip973/NVB耐药细胞株与亲代细胞Anip973细胞株端粒酶逆转录酶(human telomerase reverse transcriptase, hTERT)的mRNA表达及差异,探讨端粒酶与耐药的相关性。方法采用实时荧光定量RT-PCR法检测人肺腺癌Anip973/NVB耐药细胞与Anip973亲代细胞经NVB处理前后hTERT mRNA的表达。结果对照组Anip973、Anip973/NVB细胞的hTERT mRNA表达无统计学差异;NVB处理组Anip973细胞hTERT mRNA表达明显降低(P < 0.01),而Anip973/NVB细胞hTERT mRNA表达的降低与对照组比较无统计学差异(P > 0.05),NVB处理组Anip973细胞hTERT mRNA表达降低的程度明显高于Anip973/NVB细胞(P < 0.01)。结论端粒酶与肺腺癌耐药细胞的多药耐药密切相关,端粒酶有可能作为逆转耐药的新靶点。
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