Expression of circadian gene Per2 and VEGF in NSCL tissue and its clinical significance

Abstract

Objective To detect the expression of Per2 and VEGF in NSCLC tissue,and analyze its clinical significance.Methods The expression of Per2 and VEGF was measured in 60 NSCLC and 20 normal lung tissue by immunohistochemistry assay,then the relation with clinicopathological parameters was analyzed.Results The positive expression rate of Per2 in NSCLC and in normal lung tissue were 71.7％ and 95.0％ ( x2 =4.683,P ＜0.05 ),while the positive expression rate of VEGF were 58.3％ and 15％ (x2 =11.295,P ＜ 0.01 ).The negative expression of Per2 in NSCLC was correlated with pathological differentiation or TNM stage( x2 =4.413,6.179,all P ＜0.05),while the positive expression of VEGF in NSCLC was correlated with pathological differentiation or lymphatic metastasis (x2 =6.524,P ＜ 0.05 ).The expression of Per2 was negatively correlated with the expression of VEGF in NSCLC (r =-0.381,P ＜ 0.01 ).Conclusion The overexpression of VEGF in NSCLC may contribute to invasion and metastasis,while Per2 may be a beneficial prognosis factor in patients with NSCLC. Key words: Cancer, non-small-cell lung; Circadian gene Per2; Vascular endothelial growth factor; Immunohistochemistry