Expression and Clinical Significance of Id-1 ,-3 in Cervical Intraepithelial Neoplasia and Cervical Sqamous Cell Cancer

Abstract

Objective To explore expression and clinical significance of Id-1 ,-3 in cervical intraepithelial neoplasia (CIN) and cervical sqamous cell carcinoma (CSCC). Methods Pathological tissue samples of CIN(n = 22) and CSCC(n = 67) in Department of Pathology, First Affiliated Hospital of Fujian Medical University, which were confirmed diagnosis by histopathological examination were collected. By SP immunohistochemistry staining using the EnVision method to detect levels of Id-1, -3 protein in tissue samples. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Affiliated Hospital of Fujian Medical University. According to International Federation of gynecology and Obstetrics (FIGO) clinical stage standard, among 67 CSCC samples, they were ⅠA (4 cases), ⅠB (29 cases) , ⅡB (28 cases), ⅡB (6 cases) , respectively. Among 22 cases CIN samples, they were CIN Ⅰ (3 cases), CIN Ⅱ (5 cases), and CIN Ⅲ (14 cases), respectively. Rusults There had significance difference between tissue samples of CIN Ⅲ (n= 14) and CIN Ⅰ-Ⅱ (n = 8) in levels of Id-1, -3 protein(P 40 years old, CSCC of FIGOⅢ stages, no complicating vascular invasion, no complicating lymph node metastasis (P 0.05) ．There had significance difference of levels of Id-3 protein between complicating lymph node metastasis and those no complicating lymph node metastasis (P 0.05) . Conclusions Id-1,-3 are expression in both tissues of CIN and CSCC. Expression levels of Id-1,-3 have related with CIN grades and development of CSCC, such as invasion or metastasis, especially with invasion and metastasis. Id-1 ,-3 may be as targets of gene for therapy of CSCC. Key words: Cervical intraepithelial neoplasia; Neoplasm, sqamous cell; Cervical neoplasm, primary; Cervix uteri; Id-1; Id-3