Expression and clinical significance of fragile histidine triad gene and inhibitor of apoptosis protein in cervical tissue

Abstract

Objective To study the expression of fragile histidine triad (FHIT)and.survivin in normal cervix, cervical intraepithelial neoplasia(CIN) and cervical cancer. To discuss the potential involvement of FHIT and survivin in tumorigenesis, metastasis in cervical cancer; to discuss the significance of FHIT and survivin in early diagnosis of cervical cancer. Methods The expressions of FHIT protein and survivin were detected by immunohistochemistry in 20 cases of normal cervix, 20 cases of CIN Ⅰ ,25 cases of CIN Ⅱ/Ⅲ ,60 cases of cervical cancer. Results The percentages of negative expression of FHIT protein in normal tissues were 0% (0/20),24.4% ( 11/45 ) in CIN group, 10.0% (2/20) in CIN Ⅰ group, 36.0% ( 9/25 ) in CIN Ⅱ / Ⅲ group and 66.7%(40/60) in cervical cancer group. There were significant differences between groups (Chi-square test, P ＜0.05 ). The positive expression rate of survivin wase 20.0% ( 4/20 ) in normal group, 31.1% ( 14/45 ) in CIN group,and 95.0% (57/60)in cervical cancer group. There was significant difference between groups (Chisquare test, P ＜ 0.05 ). FHIT protein expression and survivin expression were negatively related ( P＜ 0.05 ) in CIN and cervical cancer. Conclusion Since the expression level of FHIT is associated with the grade of CIN, it can be used to screen or forecasting higher-grade-group of CIN. FHIT and survivin has clinical reference value for screening and early diagnosis of cervical lesions and cervical cancer. Key words: Cervical intraepithelial neoplasia (CIN)Cervical carcinoma; Fragile histidine triad (FHIT); Survivin; Immunohistochemistry; Early diagnosis