Expression and correlation of human papillomavirus and C-X-C chemokine receptor 7 in cervical cancer and intraepithelial neoplasm

Abstract

Objective To investigate the correlation between human papillomavirus (HPV) -16, -18 and C-X-C chemokine receptor (CXCR) 7 in cervical cancer and intraepithelial neoplasm. Methods A total of 59 patients who underwent cervix resection and were diagnosed as cervical cancer by pathological detection, 86 patients who under went cervix resection and were diagnosed as cervical intraepithelial neoplasm (CIN) by pathological detection and 20 patients who underwent cervix resection due to other diseases were collected as study subjects in the Zhuhai People′s Hospital from March 2011 to March 2013. They were enrolled into cervical cancer group (n=59), CIN group (n=86) and control group (n=20), respectively. The expression of CXCR7 was examined by immunohistochemical, and the difference of CXCR7 expression among 3 groups were analyzed. Subsequently, the relative expression of HPV-16 DNA and HPV-18 DNA were verified with quantitative real-time PCR. Eventually, the protein expression profile of CXCR7 and stromal cell-derived factor (SDF)-1 were tested by Western blotting among 3 groups, and then the correlation analysis were performed between the expression HPV-16 DNA, HPV-18 DNA and CXCR7. There were no significant differences between two groups in age and other general clinical data (P>0.05). Results ①The expression positive rates of CXCR7 in cervical tissues of cervical cancer group and CIN group both were significantly higher than that of control group, which were 88.1% (52/59) vs 15.0% (3/20) and 46.5% (40/86) vs 15.0% (3/20), and both the differences were statistically significant (χ2=37.77, P<0.001; χ2=6.68, P=0.010). ②The expression of HPV-16 DNA in CIN Ⅰ, CIN Ⅱ, CIN Ⅲ and cervical cancer tissues were (1.50±0.05) times, (2.87±0.09) times, (3.17±0.12) times and (6.41±0.20) times as much as that in normal cervix tissues, respectively, and the expression of HPV-16 DNA in CIN Ⅲ and cervical cancer tissues both were significantly higher than that in normal cervix tissues, and both the differences were statistically significant (t=2.15, P=0.042; t=3.11, P=0.003). Likewise, the expression of HPV-18 DNA in CIN Ⅰ, CIN Ⅱ, CIN Ⅲ and cervical cancer tissues were (1.56±0.07) times, (2.45±0.11) times, (3.89±0.16) times and (6.12±0.17) times as much as that in normal cervix tissues, respectively, and the expression of HPV-18 DNA in CIN Ⅲ and cervical cancer tissues both were significantly higher than in normal cervix tissues, and both the differences were statistically significant (t=2.21, P=0.024; t=3.03, P=0.004). ③The expression of CXCR7 and SDF-1 increased as the the deterioration of the disease by Western blotting. Not only that, there were positive correlation between CXCR7 expression and HPV-16 DNA (r=0.74, P<0.001), and HPV-18 DNA (r=0.78, P<0.001). Conclusion HPV may contribute the progression of cervical cancer induced by high-risk HPV. Key words: Receptors, chemokine; Human papillomavirus 16; Human papillomavirus 18; Uterine cervical neoplasms; Cervical intraepithelial neoplasia