Abstract
Cajanus cajan, a well-known legume crop with nutritional significance, has been used traditionally for various therapeutic purposes. Despite its ethnomedicinal importance and rich metabolite composition, there have been limited investigations into the secondary metabolites present in its stems using advanced analytical techniques. This study aimed to enhance our understanding of the chemical composition and biological activities of underutilized C. cajan stems. GC-MS & LC-MS analysis revealed flavonoids as the stem's predominant secondary metabolites. The methanol extract exhibited potent antibacterial activity against E. coli, S. aureus, and C. albicans. Major bioactive compounds, including rutin, apiin, quercetrin, and aesculine, contributed to these beneficial effects. The study also revealed a high total phenolic content of 121.5 mg GAE/g in the stem extract, further establishing C. cajan stems as a valuable source of therapeutic molecules.
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