Exploratory development of rapid immunohistochemical staining technique in prostate biopsy tissue frozen section diagnosis

Abstract

Objective To investigate the application value in the diagnosis of prostate biopsy tissue frozen section with rapid immunohistochemical detection of p40(ΔNp63),34βE12,p504s. Methods The expression of p40, 34βE12,p504s in 118 cases of prostate biopsy tissue frozen section detected by Max Vision rapid immunohistochemical staining technique. Conventional paraffin section immunohistochemistry of frozen remaining tissue served as a control. Results 118 cases of prostate biopsy histopathological diagnosis was divided into 79 cases of benign prostatic hyperplasia and 39 cases of prostate cancer. Max Vision rapid immunohistochemical staining technique was completed in 30minutes.Antibody expression located accurately. Background stained clearly and no interfering signal.The background was better than conventional immunohistochemistry of frozen remaining tissue. The positive expression rate of p40,34βE12,p504s with rapid immunohistochemistry in prostatic hyperplasia was 97.4%(77/79),93.7%(74/79), 0%(0/79),and in prostatic carcinoma was 0%(0/39),0%(0/39),97.4%(38/39).The positive expression rate of p40,34βE12,p504s with conventional immunohistochemistry in prostatic hyperplasia was 96.2%(76/79),93.7%(74/ 79),2.5%(2/79),and in prostatic carcinoma was 0%(0/39),0%(0/39),92.3%(36/39).The difference of expression between prostatic hyperplasia and prostatic carcinoma with rapid immunohistochemical detection p40 group:χ2= 109.402,P=0.000,34βE12 group:χ2=97.971,P=0.000,p504s group:χ2=113.537,P=0.000;The difference of expression between prostatic hyperplasia and prostatic carcinoma with conventional immunohistochemical detection p40 group:χ2=105.410,P=0.000,34βE12 group:χ2=97.971,P=0.000,p504s group:χ2=96.388,P=0.000;The expression of prostatic hyperplasia with between rapid immunohistochemical detection and conventional immunohistochemical detection 34βE12 group was identical,p40 group:χ2=0.207,P=0.649,p504s group:χ2=2.026,P=0.155; The expression of conventional immunohistochemical detection with between rapid immunohistochemical detection and conventional immunohisto-chemical detection p40 group and 34βE12 group were identical,p504s group:χ2=1.054, P=0.305.The expression of three markers between prostatic hyperplasia and prostatic carcinoma had statistical significance(P 0.05). Conclusion Max Vision rapid immunohisto-chemical staining technique has the advantages of rapid, accurate, timely. It could be used to rapid diagnosis of prostate biopsy tissue.The combined detection of p40,34βE12,p504s has very high practical value in the differential diagnosis of benign and malignant lesions of the prostate. Key words: Rapid immunohistochemistry; Prostate; p40; 34βE12; p504s; Diagnosis