Experimental study on the protective effect of salvia miltiorrhiza on myocardial lsehemia reperfusion Injury in rats

Abstract

Objective To observe the protective effect of Salvia miltiorrhiza on myocardial ischemia reperfu-sion injury in rats.Methods 150 healthy male rats were randomly divided into 5 groups：sham operation group,model group,Radix Salviae Miltiorrhizae in high dose group（100mg/kg）,middle dose group（30mg/kg）,low dose group （10mg/kg）.Salvia miltiorrhiza group were administered orally preoperatively,once a day.On 3 consecutive days. Sham operation group and model group were treated with equal volume of saline.The coronary artery ligation rat for 30min,then sonza coronary artery for 180 min to make the model of myocardial ischemia reperfusion injury.Make determination of myocardial infarct size;make determination of serum creatine kinase （ CK ） , lactate dehydrogenase （ LDH） .Results In the model group compared with the sham operation group,myocardial infarction and serum CK and LDH activity significantly increased（t =14.382,21.460,all P〈0.05）,made up of the myocardial ischemia reperfusion injury in rats model.The different doses of Danshen group can significantly reduce the myocardial ischemia reperfusion injury in rats about the range of myocardial infarction and decrease serum CK , there were significant＆amp;nbsp;differences compared with the model group （t=7.426,6.891,11.274,all P〈0.05）.Different doses of Danshen group can significantly reduce serum LDH, there were significant differences compared with the model group （ t =22.436,10.843,16.252,all P〈0.05）.Conclusion Salvia miltiorrhiza has oxidative stress on myocardial ischemia reperfusion in rats,which has protective effect of Salvia miltiorrhiza on myocardial ischemia reperfusion injury in rats. Key words: Salvia miltiorrhiza; Myocardial ischemia reperfusion injury; Protective effect; Pharmacological experiment