Experimental Studies on the Effect of Chlorpyriphos and Lead Acetate on Biochemical Parameters in Wistar Rats with Special Reference to Ameliorative Effect of Vitamin C

Abstract

The present study was designed to evaluate the effects of chlorpyriphos, lead acetate, vitamin c alone, and in combination on various biochemical parameters in wistar rats. Rats of 150–200 g body weight were divided into eight groups of six animals each and were subjected to various daily oral treatment regimes for 98 days. Group I served as control receiving only corn oil, group II received chlorpyriphos at 5.5 mg/kg in corn oil, group III received lead acetate at 100 ppm in water, whereas animals in group IVth received a combination of chlorpyriphos at 5.5 mg/kg in corn oil and lead acetate at 100 ppm in water. Group Vth received vitamin C at 100 mg/kg in water, group VIth received a combination of chlorpyriphos at 5.5 mg/kg and vitamin C at 100 mg/kg, group VIIth received lead acetate at 100 ppm in water and vitamin C at 100 mg/kg and group VIIIth received chlorpyriphos at 5.5 mg/kg, lead acetate at 100 ppm in water and vitamin C at 100 mg/kg. Chlorpyriphos and lead acetate alone or in combination elevated the activities of plasma aminotransferases, phosphatases, creatinine and blood urea nitrogen where as decreased the total plasma protein levels both due to chlorpyriphos and lead acetate treatment alone or in combination. The results showed that alterations in the biochemical parameters induced by co-exposure to chlorpyriphos and lead were mitigated by pretreatment with vitamin C. It is recommended that further research be geared towards identifying more agents that may ameliorate such adverse effects.