Abstract
Mesenchymal stem cells (MSCs) are attractive candidates for cell-based tissue repair approaches. Hundreds of clinical trials using MSCs have been completed and many others are still being investigated. For most therapeutic applications, MSC propagation in vitro is often required. However, ex vivo culture condition is not fully physiological and may affect biological properties of MSCs including their regenerative potential. Moreover, both cell cryopreservation and labelling procedure prior to infusion may have the negative impact on their expected effect in vivo. The incidence of MSC transformation during in vitro culture should be also taken into consideration before using cells in stem cell therapy. In our review, we focused on different aspects of MSC propagation that might influence their regenerative properties of MSC. We also discussed the influence of different factors that might abolish MSC proliferation and differentiation as well as potential impact of stem cell senescence and aging. Despite of many positive therapeutic effects of MSC therapy, one has to be conscious about potential cell changes that could appear during manufacturing of MSCs.
Highlights
Mesenchymal stem cells (MSCs) are one of the most exploited cell types in regenerative medicine of many clinical applications
One has to be aware that despite of great potential for application in therapeutic approach, MSCs demonstrate a number of limitations
The bothering issue is that the protocols for isolation and expansion of MSCs vary widely between laboratories, which may significantly influence the effectiveness of stem cell therapy
Summary
Mesenchymal stem cells (MSCs) are one of the most exploited cell types in regenerative medicine of many clinical applications. It is important to bear in mind that MSC is the heterogeneous cell population with different regenerative properties that may affect the final result of stem cell treatment. It is already known that the population of MSCs obtained from older or diseased patients is different in comparison to young and healthy people. Due to the infrequent presence of MSCs within tissues, in vitro propagation is almost always required to achieve a sufficient cell number for in vivo application. In vitro cell multiplication is not neutral for MSC biological properties. We point out the aspects of MSC labelling that should be applied with caution and consideration of its influence on the labelled cells
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
