Experimental Evaluation of the Efficacy of Sodium Selenite as a Remedy for Wounds

Abstract

Relevance. Emergencies, both of natural and anthropogenic origin, are accompanied by a large number of injuries and wounds, the treatment of which is associated with great difficulties due to frequent infectious complications and lethargic repair of damaged tissues. Promising approach to this problem is the use of antioxidants to reduce the severity of inflammation and stimulate repair. Intention. To evaluate the efficacy of topical application of the antioxidant composition based on selenium for acceleration of reparative processes in woundу. Methods. Experimental studies were carried out on 45 male Wistar rats. Wounds in rats were modeled under ether anesthesia by up to 4 cm excision of a pre-depilated area of the skin of the back to the underlying fascia. Wounds were treated by daily dressings with 0.5 % sodium selenite vs dimethyl sulfoxide solvent as a comparator. The presence and nature of the inflammatory reaction, the condition of edges and bottom of wounds, time to cleaning out necrotic tissues and the appearance of granulation, the nature of granulation tissue, time to the onset of epithelialization of wounds and the general condition of animals, the cytogram of wound prints, and the activity of antioxidant protection enzymes were evaluated in rats. Results and Discussion. It has been established that local use of sodium selenite has a stimulating effect on reparative processes in the wound due to increased activity of antioxidant enzymes. Thus, after 6-day treatment. sodium selenite increased catalase activity by 18.1 %, glutathione peroxidase by 63.2 %, glutathione reductase by 46.1 % compared to ointment with dimethyl sulfoxide. It was revealed that enhanced antioxidant protection of wound tissues accelerates maturation of granulations and wounds heal 1.6 times faster than in the control group. Conclusion. Medicinal compositions with sodium selenite are promising for local treatment of wounds.