Exopolysaccharides of lactic acid bacteria as protective agents against bacterial and viral plant pathogens

Abstract

In this study, 25 exopolysaccharides produced by lactic acid bacteria (LAB) were screened for their effect on plant pathogens. The molecular masses of EPS were found to be 3,8-5,0 × 104 Da. The GC–MS analysis revealed that EPSs were majorly composed of glucose (85.85–97.98 %). The FT-IR spectra of EPSs were in agreement with the typical absorption peaks of polysaccharides. EPSs showed a hydroxyl radical scavenging ability. The scavenging rate of EPS ranged from 20 to 50 % at a concentration of 5.0 mg/mL. Significant growth delay of phytopathogenic bacteria was observed after 3–6 h of cultivation. Optical density values of indicator cultures growing in the medium with EPS (1 mg/mL) were lower compared to the control by 24–100 % for Pseudomonas fluorescens, 9–46 % for P. syringae, 47–79 % for Pectobacterium carotovorum, 14–90 % for Clavibacter michiganensis, 9–100 % for Xantomonas campestris, and 45–100 % for X. vesicatorium. EPS retained their inhibitory effect on the growth of X. campestris, X. vesicatorium and C. michiganensis strains after 24–48 h of cultivation, but stimulating effect on the growth of some strains also was observed. LAB EPS showed antibiofilm activity against P. carotovorum, P. syringae, and P. fluorescent, decreasing their biofilm formation by 16–50 %, 14–39 %, and 29–59 %, respectively. Also, stimulation of biofilm formation by X. campestris (by 8–29 %), X. vesicatorium (by 3–32 %) and C. michiganensis (by 31–41 %) strains was observed. EPSs showed antiviral activity against tobacco mosaic virus (TMV). At a concentration of 100 μg/mL, they decreased the infective ability of TMV by 61–92 %. This is the first study demonstrating that LAB EPS exhibited in vitro antibacterial and antibiofilm activity against phytopathogenic bacteria and anti-viral activity against TMV. Thus, LAB EPSs could have great potential for plant protection strategies.