Abstract
The colicinogenic factor ColE 1 has previously been isolated as a supercoiled circular DNA-protein complex designated relaxation complex. When treated with proteases, strong ionic detergents, or alkali, the supercoiled DNA in the complex is converted to the relaxed, or open circular, form. In these studies the colicinogenic factor-sex factor ColI b -P9 has been purified from Escherichia coli as a circular DNA molecule with sedimentation values of 73S and 45S for the supercoiled and open circular forms, respectively, and as a supercoiled DNA-protein relaxation complex with properties similar to those described for the ColE 1 relaxation complex.
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