Abstract
This study was designed to examine the effects of acute exercise, insulin stimulation, and their combination on the kinetics of glucose transport in rat skeletal muscle. Sarcolemmal (SL) membranes were isolated from control (C), acute exercise (E), insulin-stimulated (I), and combined (E + I) rats. Michaelis-Menten kinetics indicated that the Vmax for glucose transport was increased after each perturbation compared with C but were not different from each other (E, 4,334 +/- 377; I, 4,424 +/- 668; E + I, 4,338 +/- 602; and C, 1,366 +/- 124 pmol.mg protein-1.s-1). The apparent Km was unchanged. Scatchard plots of cytochalasin B binding sites indicated that both I and E + I increased the number of binding sites compared both E and C (9.4 +/- 0.5 and 7.8 +/- 0.5 vs. 5.1 +/- 0.2 and 5.5 +/- 0.3 pmol/mg protein) without altering the dissociation constant. The increase in Vmax was greater than the increase in cytochalasin B binding sites indicating that both I and E + I caused an increase in the turnover rate of transport molecules as well as an increase in the total number of transport molecules. Because there was no change in the Km for glucose transport and no increase in cytochalasin B binding sites after exercise, the increase in Vmax was due solely to an increased turnover rate of existing transport molecules.
Published Version
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