Abstract
Abstract Human β2-microglobulin (β2m) can be exchanged in mouse histocompatibility (H-2) antigens for native β2m The exchange can be achieved at the cell surface by incubating mouse cell suspensions in medium containing free β2m, or in cell free suspensions using purified H-2 antigens. The complexes containing radiolabelled human β2m and H-2 antigens can be immunoprecipitated specifically with alloantisera and with monoclonal anti H-2 antibodies. Specific association of human β2m with H-2 antigens was observed with several haplotypes including H-2. Radiolabelled H-2 antigens associated with unlabelled human β2m can be precipitated with monoclonal antibody against human β2m. Thus radiolabelled human β2m can be used as a specific label for mouse H-2 antigens in precipitation and radioimmunoassays, and monoclonal anti-human β2m antibody can be used for affinity purification of H-2 antigens. The application of this method of exchange can be extended to major histocompatibility complex (MHC) antigens of other species, and to MHC linked differentiation antigens having a natural association with β2m.
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