Abstract
Excess single-stranded DNA inhibits meiotic double-strand break repair
Highlights
In most organisms the success of meiosis is dependent on the creation of molecular joints that serve to lock homologous chromosomes together until they mediate ordered chromosome segregation at the first meiotic division
Both sister chromatids containing the VMA1-derived endonuclease (VDE)-recognition sequence are usually cleaved during meiosis, and tetrad analysis revealed both chromatids are frequently repaired to Dproduct, an outcome most compatible with repair by single-strand annealing (SSA) [31]
To further determine how the presence of Spo11-double-strand break (DSB) can influence VDE-DSB repair in trans, we have investigated two VDEDSB sites in dmc1D meiotic cells
Summary
In most organisms the success of meiosis is dependent on the creation of molecular joints that serve to lock homologous chromosomes together until they mediate ordered chromosome segregation at the first meiotic division. This is achieved by the creation of crossovers, which creates a covalent link between nonsister chromatids, and through the forces of sister chromatid cohesion to maintain a link between chromosome pairs until first anaphase (reviewed in [1]). Because intersister repair does not create links between homologous chromosomes, meiotic cells have evolved a strong bias toward using the homologous chromosome as donor template. Much attention is currently focused on understanding how the meiotic cell enforces the preference for interhomolog repair
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call