Evaluation of Ultra-High-Performance Liquid Chromatography (HPLC) Tandem Mass Spectrometry for Determination of Avermectin Residues in Milk

O V Bayer,L V Shevchenko,O M Stupak,V M Mykhalska,S V Midyk,O I Skoromna,O V Kaminska,O S Yaremchuk,O V Bondarets,N V Liniichuk

doi:10.15421/2019_784

Abstract

The study was conducted to evaluate the applicability of ultra high-performance liquid chromatography - tandem mass spectrometry method (UHPLC-MS/MS), establish the MS/MS detection parameters and determine the validation characteristics for the analysis of residual content of avermectins in milk. The UHPLC-MS/MS method has proved to be accurate, practical and universal. This was confirmed by Decision limit (CCα) data: abamectin - 12.56 μg/kg, doramectin - 17.74 μg/kg, eprinomectin - 24.02 μg/kg, ivermectin - 12.53 μg/kg, moxidectin - 44.69 μg/kg, recovery is 88.7-110%. The data obtained for assessing the suitability, accuracy and reproducibility of the results meet the requirements of the European Directive (2002/657/EC). The efficient ultra high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method that was developed and adopted for routine use by the laboratories of veterinary medicine, allows to detect residual quantities of 5 avermectins used in animal breeding for the prevention of helminthiases in food products including milk.

Highlights

Evaluation of food products' safety lies in determination of toxic substances (Yakubchak et al, 2018) and residues of veterinary drugs used in the process of productive animal breeding
At levels of interest 10-20 μg/kg this index was rather low for ivermectin (14.2%), abamectin (13.2%), doramectin (9.9%), eprinomectin (10.6%), and moxidectin with the concentration of 40 μg/kg (6.4%)
Validation of the method of determining the residual content of avermectins in raw milk indicates that the use of UHPLC-MS/MS allows obtaining highly accurate data

Summary

Introduction

Evaluation of food products' safety lies in determination of toxic substances (Yakubchak et al, 2018) and residues of veterinary drugs used in the process of productive animal breeding. The enzyme-linked immunosorbent assay (ELISA) is used to determine the residual content of avermectins in livestock products This method is rather convenient for samples screening, for it does not involve complex sample preparation and equipment. As seen from the above, the test systems are designed exclusively for the individual matrices (eggs, muscles, milk) and do not cover the entire list of existing avermectins There is another method of detecting avermectins' residues in biological samples, which involves the usage of immunoaffinity columns (Wu et al, 2001) for samples preparation. The ultra-high-performance liquid chromatography tandem mass spectrometry method (UHPLC-MS/MS) is a confirmatory method for detecting the level of avermectins in animal tissues and milk It has higher sensitivity and encompasses wide range of analytes. Samples are typically extracted using organic solvent and cleaned up by the method of liquid-liquid extraction (LLE), which makes it more affordable, faster and more sensitive

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Conclusion