Evaluation of the preventive potential of graded dietary inclusion of Hyphaene thebaica (Linn) fruit in rat model of colon carcinogenesis.

Abstract

The preventive effect of Hyphaene thebaica fruit in colon carcinogenesis was evaluated in Wistar rats at 0, 2.5, 5 and 10% inclusion rates for twelve weeks with concomitant 72-h intra-rectal N-methyl-N-nitrosourea (MNU) instillations. Indices of antioxidant status and carcinogenesis were analyzed using spectrophotometric, ELISA, histological and immunohistochemical techniques. The fruit protected against lipid peroxidation and level of early biomarkers of colon carcinogenesis, accompanied by decrease in some endogenous antioxidant enzymes functionality. It also prevented colon tissues against MNU-induced severe inflammations and damage to the mutL-homolog 1 (MLH1) gene. There was significant negative correlation between endogenous antioxidant enzyme activities and carcinoembryonic antigen (CEA) as well as lipid peroxidation, but relationship between total polyphenols and percentage expression of MLH1 proteins as well as endogenous antioxidant enzyme activities was positive. These results validate the folkloric use of H. thebaica fruit in the management of colorectal disorders. PRACTICAL APPLICATIONS: Hyphaene thebaica fruit which is widely consumed in northern Nigeria and other countries of sub-Saharan Africa is rich in fiber and antioxidant polyphenols. These two classes of compounds have demonstrated capacity to prevent colorectal cancer and cancer of other sites. Therefore, the validated protective Hyphaene thebaica fruit suggests that it can be processed for inclusion in beverages/diets as functional foods for prevention and management of colorectal disorders.