Evaluation of the anti-inflammatory activity of N, N′-bis(3-dimethylamino-1-phenyl-propylidene)hydrazine dihydrochloride

Abstract

This study investigated the anti-inflammatory effect of N, N′-bis(3-dimethylamino-1-phenyl-propylidene)hydrazine dihydrochloride, D1, on carrageenan-induced edema. In addition, its effect on hyaluronidase-induced vascular permeability was also tested. D1 was synthesized, and anti-inflammatory activity was determined by carrageenan-induced hind paw edema in rats (n = 30) at 50, 100, and 200 mg kg−1 doses of D1 and also a 25 mg kg−1 dose of indomethacin. The effects of D1 and indomethacin on hyaluronidase-induced capillary permeability were investigated in rabbits (n = 18) at a 100 mg kg−1 dose of D1 and 25 mg kg−1 dose of indomethacin. D1 inhibited carrageenan-induced inflammation by 40, 20, and 10% at 50, 100, and 200 mg kg−1 doses after 1 h. The inhibitions were 22.5, 32.7, 28.6% and 15.6, 33.4, 8.9% at 2 h and 3 h, respectively. The inhibitions due to indomethacin (25 mg kg−1 dose) were 67.5, 87.8, and 91.1%, at 1 h, 2 h, and 3 h, respectively. The subcutaneous spreading areas of Trypan blue at 1, 5, 30, and 60 min after subcutaneous injection of hyaluronidase were 172.6 ± 41.6, 210.2 ± 39.7, 363 ± 50, and 400.2 ± 46.7 mm2 in the D1 (100 mg kg−1) treated group. The spreading areas at these time periods were 38.8 ± 3.7, 48.2 ± 4.5, 100.6 ± 6.9, and 119.8 ± 22.5 mm2 in the indomethacin treated group. Our results showed that D1 inhibits carrageenan-induced inflammation in rats. A tendency to decrease the capillary permeability suggested that the mechanism of action of the anti-inflammatory effect of D1 may partly depend on inhibition of the hyaluronidase enzyme.