Analgesic, anti-inflammatory and anti-ulcer properties of Thai Perilla frutescence fruit oil in animals.

Pimpisid Koonyosying,Duangta Kanjanapothi,Winthana Kusirisin,Narisara Paradee,Kovit Pattanapanyasat,Somdet Srichairatanakool,Rattanaporn Janthip

doi:10.1042/bsr20203166

Pimpisid Koonyosying, Duangta Kanjanapothi + Show 5 more

Open Access

https://doi.org/10.1042/bsr20203166

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Abstract

Perilla frutescens fruit oil (PFO) is rich in α-linolenic acid (ALA) and exhibits biological activities. We aimed to investigate analgesic, anti-inflammatory and anti-ulcer activities of PFO and PFO-supplemented soybean milk (PFO-SM) in animal models. Analgesic activity was assessed in acetic acid-induced writhing in mice, while anti-inflammatory activity was performed in ethyl phenylpropiolate (EPP)-induced ear edema and carrageenan-induced hind paw edema in rats. Anti-ulcer effects were conducted in water immersion stress, HCl/ethanol and indomethacin-induced gastric ulcer in rats. Distinctly, PFO, containing 6.96 mg ALA and 2.61 mg LA equivalence/g, did not induce acute toxicity (LD50 > 10 mL/kg) in mice. PFO (2.5 and 5 mL/kg) and PFO-SM (0.05 mL PFO equivalence/kg) inhibited incidences of writhing (16.8, 18.0 and 32.3%, respectively) in acetic acid-induced mice. In addition, topical applications of PFO (0.1 and 1 mL/ear) significantly inhibited EPP-induced ear edema (59.3 and 65.7%, respectively) in rats, while PFO-SM slightly inhibited ear edema (25.9%). However, PFO and PFO-SM did not inhibit carrageenan-induced hind paw edema in rats. Indeed, PFO (2.5 and 5 mL/kg) significantly inhibited gastric ulcers in rats that induced by water immersion stress (92.4 and 96.6%, respectively), HCl/ethanol (74.8 and 73.3%, respectively) and indomethacin (68.8 and 88.9%, respectively), while PFO-SM did not. PFO displayed potent analgesic, anti-inflammatory and anti-ulcer properties, while PFO-SM exerted only analgesic properties. Thus, Thai PFO and its functional drink offer potential benefits in treatment of analgesic, inflammatory diseases and gastric ulcer.

Highlights

Inflammation, which can be caused by physical and chemical trauma, drugs and infectious microorganisms, is a defense mechanism of the body that serves to eliminate damaged tissue or invading pathogens [1]
ALA and linoleic acid (LA) in Perilla frutescens fruit oil (PFO) were identified by comparison of their retention time (TR) values with those of the authentic standards, and the resulting concentrations were determined using the calibration curves obtained from different concentrations
The results imply that the median lethal dose (LD50) of PFO was estimated to be more than 10 mL/kg

Summary

Introduction

Inflammation, which can be caused by physical and chemical trauma, drugs and infectious microorganisms, is a defense mechanism of the body that serves to eliminate damaged tissue or invading pathogens [1]. Related responses are characterized by localized redness, heat, swelling, pain and a loss of tissue function. During these responses, vascular permeability is increased to recruit inflammatory cells to the injury sites, which can cause leakage of plasma fluid and subsequent edema. Edema and pain can cause a loss of tissue function. Uncontrolled inflammation contributes to a variety of inflammatory diseases including cardiovascular disease, diabetes and gastric ulcers [2,3]

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