Abstract
Cell patterning is becoming increasingly popular in neuroscience because it allows for the control in the location and connectivity of cells. A recently developed cell patterning technology uses patterns of an organic polymer, parylene-C, on a background of SiO2. When cells are cultured on the parylene-C/SiO2 substrate they conform to the underlying parylene-C geometry. Parylene-C is, however, just one member of a family of parylene polymers that have varying chemical and physical properties. In this work, we investigate whether two commercially available mainstream parylene derivatives, parylene-D, parylene-N and a more recent parylene derivative, parylene-HT to determine if they enable higher fidelity hNT astrocyte cell patterning compared to parylene-C. We demonstrate that all parylene derivatives are compatible with the existing laser fabrication method. We then demonstrate that parylene-HT, parylene-D and parylene-N are suitable for use as an hNT astrocyte cell attractive substrate and result in an equal quality of patterning compared to parylene-C. This work supports the use of alternative parylene derivatives for applications where their different physical and chemical properties are more suitable.
Highlights
Patterning of in vitro cell culture surfaces facilitates spatial control over the growth of cells
We evaluate whether parylene-HT, parylene-D and parylene-N are compatible with existing protocols for fabricating parylene/SiO2 substrates, and compare the quality of cell patterning achieved with parylene-C substrates
XPS spectra were collected from both parylene and ablated SiO2 areas of the substrates in order to verify that each parylene derivative had been completely removed
Summary
Patterning of in vitro cell culture surfaces facilitates spatial control over the growth of cells. In contrast to typical cell culture, patterned cultures offer the opportunity to better represent more complex patterns of connectivity that reflect the in vivo environment [1, 2]. Cell patterning can be achieved by tuning the physical and chemical properties of polymers so that they form cell repulsive and cell attractive surface [3,4,5]. A cell patterning technique that was recently developed by Delivopoulos et al demonstrated how geometric patterns of the polymer.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
