Evaluation of Oral Preparations of Vitamin E as Antioxidant Using DPPH Method (Diphenyl picrylhydrazyl)

Abstract

This article aimed to evaluate the activity of Vitamin E as an antioxidant in oral pharmaceutical preparations. Vitamin E is an essential nutrition soluble in fat that has the pharmacology effect as an antioxidant. Vitamin E consists of α, β, γ, δ-tocopherol and α, β, γ, δ-tocotrienol. The activity of vitamin E was observed for its ability to inhibit reduction using the DPPH method. The antioxidant activity is calculated as an inhibition concentration of 50% (IC50) which is the concentration of a sample that can reduce 50% of DPPH. It was known that IC50 of vitamin E was very strong with value of 42.86%. Based on the article review, it can be concluded that the activity of Vitamin E as an antioxidant in oral pharmaceutical preparations was very high and strong. Keywords: Vitamin E, alpha-tocopherol, antioxidant, activity, IC50, oral pharmaceutical preparations