UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOLIK KULIT BUAH JERUK NIPIS (Citrus aurantifolia) DENGAN METODE DPPH (1,1-difenil-2- pikrilhidrazil)

Abstract

ABSTRACT Citrus aurantifolia is known contains flavonoid compound and high Vitamin C. One of the effects of flavonoid and Vitamin C are antioxidant. This research aims to knowing the antioxidant activity of citrus aurantifolia etanolic extract by using the DPPH (1, 1-difenil-2-pikrilhidrazil) method and to knowing the active compound that containing whithin. The research was done by making level series of citrus aurantifolia etanolic extract, they were 10, 20, 40 and 80 μg/ml. As a standard of comparison was used vitamin C with concentrations 1, 2, 4 and 8 μg/ml. As a blank was used DPPH 0,1 mM. The antioxidant activity test was done by the DPPH method. The achieved data was counted to know it’s antioxidant activity. The statistical analysis was used T-Test. To know IC50 (Inhibition Concentration)50 was used the probit analysis and to know it’s active compound content was done an identification with TLC (Thin Layer Chromatography). The result of the research shows that etanolic extract of citrus aurantifolia has antioxidant activity IC50 about 54,458 μg/ml and 4,768 μg/ml for vitamin C. The statistical test result of antioxidant activity shows that there is no any significant difference. The TLC result shows that compound contained in etanolic extract of citrus aurantifolia are flavonoid and Vitamin C. Keywords: Citrus aurantifolia extract, DPPH method, antioxidant and IC50.