Evaluation of antioxidant and antimicrobial activity of various extracts of Buxus wallichiana Baill wood

Abstract

The present study was designed to evaluate the antioxidant and antimicrobial activity of various extracts of Buxus wallichiana Baill wood. Extraction of wood was done by hot continuous percolation process by using suitable solvents and the concentrated extract was used for the study. Anti oxidant Activity is done by in vitro DPPH (2, 2-diphenyl-1-picryl hydrazyl) method and Nitric oxide radical inhibition activity method, where Antibacterial Activity is done by Cup Plate Method. The MEBW (Methanolic extract of Buxus wallichiana) showed an IC50 value of 29.2±2.10, where as ABBW (Aqueous extract of Buxus wallichiana), CEBW(cholroform extract of Buxus wallichiana) and PEBW (Petroleum ether extract of Buxus wallichiana) produced IC50 values of 190±5.33, 647.5±16.43 and 747.5±10.2 respectively in DPPH method. When assayed for Antibacterial Activity MEBW at 500 μg/ml produced zone of inhibition of 26mm against Klebsiella aerogenes organism, against proteus vulgaris organism, CEBW, AEBW at 500 μg/ml produced zone of inhibition of 19mm. Against bacillus premiles, b. aerogenes and b.coacoagulans MEBW at 500 μg/ml shown zone of inhibition of 17, 19 and 24 mm respectively. The result of this study reveal that Buxus wallichiana Baill could be considered as potential source of natural anti oxidant and anti bacterial plant.