Evaluation of Medicinal Effects of Gynura Procumbens Leave Extracts On Oxidative, Glycemic, Lipidomics, and Enzymatic Profiles in Alloxan-Induced Diabetic Mice

Abstract

To evaluate the medicinal impact of G. procumbens, this study designed to explore the anti-oxidant, anti-diabetic, anti-lipidemic, and enzyme activities of G. procumbens leaves extract. Alloxan induced diabetic mice used for anti-diabetic activity. Blood was collected and analyzed glucose level, lipid profile, hepatic performance, renal activity, and oxidative stress emblem with different hours (1-5 hours) and days (7th -28th days) interval using standards protocol. Bioactive compounds like alkaloid, tannin, phenol, flavonoid, and terpenoid were present in the extracts. Anti-oxidant activities were evaluated at concentration of 15, 20, 25, 30, 35, 40 and 45 μg/mL but aqueous extracts showed very significant free radical scavenging activity at concentration 40 and 45 μg/mL, (**p>0.001). Both the aqueous and ethanolic extracts (GPLAE-200 and GPLEE-200) exhibited a remarkable lowering of blood glucose level, compared to the control mice. Anti-diabetic results reveal that GPLEE-200 could be applicable for acute but GPLAE-200 for chronic treatment for diabetes mellitus. Aqueous extracts of G. procumbens have significant effects on cholesterol, triglycerides (TG), HDL and LDL level but GPLEE-200 have prominent effects on lowering LDL level. GPLAE-200 exhibit significant lowering effects of SGPT, SGOT, ALP (Alkaline phosphatase) and creatinine level. whereas; GPLEE-200 has prominent lowering effects on SGOT and creatininelevel. This study may suggest that treatment of the extracts was dose-dependent, and aqueous extract of G. procumbens could be a promising resource for better treatment of diabetes.