Evaluation of In vitro antioxidant potential of active metabolite constituents of different extracts of Chaetomium cupreum-SS02 by spectrophotometric method

Abstract

Objective: The main objective of the study was to evaluate the antioxidant activities of Chaetomium cupreum extracts. Materials and Methods: The total flavonoid content was determined by using aluminum chloride method, whereas antioxidant activity (AA) was evaluated by ferric reducing antioxidant power assay, potassium ferricyanide reducing power assay, 2,2-diphenyl-1-picyl-hydrazyl method, β-carotene bleaching assay, cupric ion reducing antioxidant capacity assay, lipid peroxidation inhibition assay by thiobarbituric acid (TBA)-reactive substance method, and inhibition of hydrogen peroxide-induced erythrocyte hemolysis assay. Results: The ferric reducing AA of C. cupreum extracts at the concentration of 50 μg/mL was higher in ethyl acetate extract (6.11%) followed by chloroform extract (3.96%), n-butanol extract (2.44%), and methanol extract (2.02%) mg RE/g dry weight. The potassium ferricyanide reducing activity of C. cupreum extracts at the concentration of 50 μg/mL was higher in ethyl acetate extract (15.90%) followed by chloroform (9.50%), n-butanol (4.93%), and methanol extract (2.92%). The 2, 2-diphenyl-1-picryl-hydrazyl activity of C. cupreum extracts at 50 μg/mL was higher in ethyl acetate extract (36.13%) followed by n-butanol extract (24.17%), chloroform extract (15.04%), and methanol extract (4.71%). The β-carotene bleaching activity of C. cupreum extracts at 50 μg/mL after 1 h of incubation was higher in ethyl acetate extract at 12.88%, followed by chloroform extract (9.82%), n-butanol extract (5.63%), and methanol extract (3.76%). The cupric ion reducing AA (CUPRAC) of C. cupreum extracts at 50 μg/mL was highest in the methanol extract (18.62%) followed by ethyl acetate extract (9.72%), n-butanol extract (7.18%), and chloroform extract (2.46%) mg ACE/g dry weight. With regard to TBA reactive substance activity of C. cupreum extracts at 50 μg/mL, n-butanol extract showed the highest lipid peroxidation inhibition (55.39%) followed by chloroform extract (50.51%), ethyl acetate extract (46.27%), and methanol extract (43.60%). With regard to the hydrogen peroxide-induced hemolysis inhibition activity of C. cupreum extracts at the concentration of 500 μg/mL, ethyl acetate extract showed the highest inhibition (30.53%) followed by chloroform extract (26.42%) and n-butanol extract (9.16%). Conclusion: The results of the present study showed that C. cupreum extracts poses significant antioxidant potential.