Abstract
Investigation of phytochemical and biological activities was performed by Antioxidant (DPPH, H2O2, Nitric oxide) free radical scavenging methods against three gradient polarity solvents extracts of leaves of Melia azaradach Linn with different concentrations. MA species of three different assay (DPPH, H2O2, Nitric oxide) of anti-oxidant activity were showed to be IC50 values of 20.14, 35.98, 36.43 with methanolic extract of leaves were have higher concentration possess more antioxidant potential than MALEoC IC50 42.85, 54.18, 30.56 and MALEoH IC50 values of 44.68, 52.71, 48.05 when compare to reference standard ascorbic acid IC50 values of 17.87, 18.91, 25.83. They exhibited strong antioxidant DPPH, H2O2, Nitric oxide radical scavenging activities with IC50 value reference ascorbic acid, MALoME, MALoCE and MALoHE respectively. Phytoconstituents like carbohydrates, flavonoids, proteins, saponins and tannins etc. An antioxidant activity of methanol extract could be due to the presence of flavonoids and phenols. The results suggest that methanol and chloroform extracts from leaf melia azedarach tree have strong antioxidant potential.
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