​Evaluation of Homologous Platelet Rich Plasma (PRP) Drop and Leucocyte Platelet Rich Fibrin (L-PRF) Membrane for Corneal Ulcer Healing in Dogs

Abstract

Background: To evaluate use of homologous platelet rich plasma (PRP) drop and leucocyte-platelet rich fibrin (L-PRF) clot/membrane as regenerative therapy for healing of corneal ulcer in dogs and its subsequent comparison with conventional therapy. Methods: In this randomized study, 30 dogs were divided into three groups of 10 animals each. It was ensured each group had grade I, II and III ulcers. Group I received medicinal treatment with antimicrobial and non-steroidal anti-inflammatory eye drop. In Group II PRP drops and in Group III L-PRF membrane was used to evaluate corneal ulcer healing. Homologous platelet concentrates were prepared in laboratory and used for assessment of healing on various grades of corneal ulcer on basis on reduction in size and depth of ulcer, presence of stromal infiltration, scleral involvement, corneal opacity and vascularization. Result: Post treatment, the size of corneal ulcer started to reduce gradually from day 7 onwards. Significant (p≤0.05) reduction was observed from day 10 in group I after instillation of drops Moxifloxacin and Flurbiprofen and in group II after instillation of PRP. It was measured as 5.35 ± 0.63 and 5.20 ± 0.98 mm at day 0, which reduced significantly (p≤0.05) to 3.90 ± 0.50 and 3.10 ± 0.98 mm at day 10 in group I and group II respectively. In group III there was significant reduction in size of ulcer from day 0 to day 7 measuring 7.80 ± 1.05 and 0.20 ± 0.11mm respectively. Thereafter, from day 10 onwards complete sealing of ulcer was observed in all the cases. Post treatment there was no significant reduction in corneal opacity from day 0 to day 30 in group I dogs and corneal opacity persisted specially in grade II and grade III ulcers. There was significant reduction (p≤0.05) in opacity from 2.90 ± 0.35 to 2.10 ± 0.38 and 3.00 ± 0.37 to 2.40 ± 0.34 in group II and group III dogs from day 0 to day 10 respectively. In group I, II and III there was significant (p≤0.05) reduction in vascularization from 1.50±0.30 to 1.20±0.13, 1.30±0.37 to 0.60±0.22 and 1.40±0.31 to 0.40±0.31 from day 0 to day 15 respectively. There was a significant reduction in vascularization on day 15 in group II and group III compared to group I animals.