Homologous Platelet-Rich Plasma and Leucocyte–Platelet-Rich Fibrin as a Regenerative Therapy for Corneal Ulcers

Abstract

AbstractIn this prospective, randomized study discussed in this chapter, 30 dogs were randomly divided into 3 groups of 10 animals each to evaluate the use of homologous platelet-rich plasma (PRP) drop and leucocyte–platelet-rich fibrin (L–PRF) clot/membrane as a regenerative therapy for the corneal ulcer healing in dogs and its subsequent comparison with conventional therapy. It was ensured that each group had grade I, II, and III ulcers. Group I animals received conventional medical treatment of antimicrobial and non-steroidal anti-inflammatory eye drop. This group acted as the control. In Group II animals PRP drops and in Group III animals L–PRF membrane were used to evaluate corneal ulcer healing. Homologous platelet concentrates were prepared in the laboratory and used for assessment of healing on various grades of corneal ulcer on the basis of reduction in size and depth of ulcer, presence of stromal infiltration, scleral involvement, corneal opacity, and vascularization. No adverse reaction was found during regenerative therapy of corneal ulcer using homologous L–PRF membrane and PRP drop. On the basis of various antimicrobial analyses, both PRP and L–PRF showed significantly (p < 0.05) greater antimicrobial activity when compared with moxifloxacin. Based on clinical parameters, homologous L–PRF membrane showed significantly earlier clinical healing followed by PRP drop and conventional treatment of moxifloxacin and flurbiprofen.Key wordsRegenerative therapyPlatelet-rich plasmaLeucocyte–platelet-rich fibrinCorneal ulcer/ulcerative keratitisCorneal opacityCorneal vascularization