Abstract
Stability of pharmaceutical products is the ultimate objective of the pharmaceutical sector. Preservatives are the essential and effective part of all types of Syrup and suspension formulations. A fast reversed-phase high performance liquid chromatography method is developed for the determination of sodium benzoate (SB) and alkyl esters of Para hydroxyl benzoic acid such as ethyl paraben (EP), methyl paraben (MP), and propyl paraben (PP) in marketed analgesic suspensions collected from different sources. Complete separation of all the analytes was achieved on an Hypercil C18 (256 mm × 6.4 mm; 5 µm) column using acetonitrile and acetate buffer (pH 5) in the ratio of (35:65, v/v) as the mobile phase pumped at a flow rate of 1.5 mL/min in isocratic mode. Under the optimized experiment conditions, separation of the analytes was achieved in less the 10 min. Efficacy of the preservatives is determined in the prepared suspensions using the identified strains of bacteria, that is, Escherichia coli ATCC (8739), Pseudomonas aeruginosa ATCC (9027), and Staphylococcus aureus ATCC (6538) for a period of 28 d.
Published Version
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