Evaluation of bioactive secondary metabolites from endophytic fungus Pestalotiopsis neglecta BAB-5510 isolated from leaves of Cupressus torulosa D.Don.

Deeksha Sharma,Pavan Kumar Agrawal,Avijit Pramanik

doi:10.1007/s13205-016-0518-3

Deeksha Sharma, Pavan Kumar Agrawal + Show 1 more

Open Access

https://doi.org/10.1007/s13205-016-0518-3

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Journal: 3 Biotech	Publication Date: Sep 29, 2016
Citations: 175	License type: CC BY 4.0

Affiliation: Central University of Haryana

Abstract

Six endophytic fungi were isolated from Cupressus torulosa D.Don and identified phenotypically and genotypically. The fungal cultures were further grown and the culture was extracted by two organic solvents methanol and ethyl acetate. The screening was carried out using the agar well diffusion method against human pathogen such as Escherichia coli, Salmonella typhimurium, Bacillus subtilis and Staphylococcus aureus. Isolated strain of Pestalotiopsis sp. was showing prominent antibacterial activity. The crude methanol and ethyl acetate extract of Pestalotiopsis sp. showed MIC of 6.25 mg/mL for S. typhimurium and S. aureus which showed its efficacy as a potent antimicrobial. The phytochemical screening revealed the existence of a diverse group of secondary metabolites in the crude extracts of the endophytic fungi that resembled those in the host plant extracts. On the basis of phenotypic characteristics and rDNA sequencing of the ITS region of the endophyte was identified as P. neglecta which turned out to be a promising source of bioactive compounds. There is little known about endophytes from C. torulosa D.Don. In this paper we studied in detail the identification of isolated endophytic fungi P. neglecta from C. torulosa D.Don and characterization of its active metabolite compounds. The partially purified second fraction (PPF) extracted from the fungal culture supernatant was subjected to gas chromatography followed by mass spectrometry which revealed the presence of many phytochemicals. These results indicate that endophytic fungi P. neglecta isolated from medicinal plants could be a potential source for bioactive compounds and may find potential use in pharmaceutical industry.

Highlights

An increase in the number of people in the world having health problems caused by various cancers, drug-resistant bacteria, parasitic protozoans, and fungi is a cause for alarm (Strobel 2003)
The crude methanol and ethyl acetate extract of Pestalotiopsis sp. showed minimum inhibitory concentration (MIC) of 6.25 mg/mL for S. typhimurium and S. aureus which showed its efficacy as a potent antimicrobial
In this paper we studied in detail the identification of isolated endophytic fungi P. neglecta from C. torulosa D.Don and characterization of its active metabolite compounds

Summary

Introduction

An increase in the number of people in the world having health problems caused by various cancers, drug-resistant bacteria, parasitic protozoans, and fungi is a cause for alarm (Strobel 2003). Development of multiple drug-resistant microbes raised the need to search for new and novel antimicrobials for treatment of human diseases (Wise 2008). An intensive search for newer and more effective agents to deal with these disease problems is under way and endophytes are a novel source of potentially useful medicinal compounds. Microorganisms have the ability to utilize various substrates as a consequence of the diversity of their biological and biochemical evolution (Fernandes et al 2009). The solid substrates they use include, among others, live plants. Both bacteria and fungi are known to cooperate with many plants to form mutually beneficial associations. Actinomycetes and fungi, of all microorganisms studied, have been found to be the most prolific producers of secondary metabolites (Guanatilaka 2006)

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