Abstract
In Nigeria, rural inhabitants often resort to herbal remedies and dietary control for the treatment and management of various forms of diabetes mellitus. This study was conducted to provide the rationale for the use of Psidium guajava leaves as a potent traditional anti-diabetic remedy. The crude leaf extracts of n-hexane, methanol, and ethyl acetate of Psidium guajava were separately prepared by cold maceration. Then, ethyl acetate crude extract of Psidium guajava leaves was fractionated by column chromatography to yield ethyl acetate bulked fractions: EF-I (1-75), EF-II (76-150), and EF-III (151-250). The n-hexane, ethyl acetate, and methanol crude leaf extracts and ethyl acetate bulked fractions (EF-I, EF-II and EF-III) were evaluated for anti-diabetic activity in alloxan-induced diabetic mice. The blood sugar levels of treated and untreated alloxan-induced diabetic mice were assayed as indices of anti-diabetic effect. The phytochemical constituents of both crude extracts and ethyl acetate fractions of Psidium guajava leaves and the mean lethal dose (LD50) of ethyl acetate crude leaf extract of Psidium guajava were determined. The mean lethal dose (LD50) of ethyl acetate crude leaf extract was calculated to be 1500mg/kg b.w. The results indicated that oral administration of ethyl acetate, n-hexane, methanol crude extracts, and ethyl acetate bulked fractions of Psidium guajava leaves at a dose of 100mg/kg b.w on treated groups exhibited much significant[p<0.001, p<0.01and p<0.05]anti-hyperglyceamic effect by ameliorating high blood sugar levels of alloxan-induced diabetic treated mice, while EF-II and EF-III showed non-significant[p>0.05] anti-hyperglyceamic activity for the reduction in blood sugar levels compared with the negative and positive control groups. The anti-diabetic potency of the crude leaf extracts and ethyl acetate fractions were in the order; EC>HC>MC>EF-I>EF-II>EF-III. The results of phytochemical screening of the crude extracts and ethyl acetate bulked fractions showed the presence of tannins, flavonoids, saponins, alkaloids, terpenoids, glycosides, and steroids while reducing sugar was absent. The results from this study gives credence to the use of Psidium guajava as an anti-diabetic agent in the management of diabetes mellitus.
Highlights
Diabetes mellitus elucidates a metabolic disease characterized by abnormal hyperglycemia which alters the metabolism of lipids, carbohydrates, proteins arising from insulin-deficiency or insensitivity of target cell to insulin secreted in the body [37]
The results indicated that oral administration of ethyl acetate, n-hexane, methanol crude extracts, and ethyl acetate bulked fractions of Psidium guajava leaves at a dose of 100mg/kg b.w on treated groups exhibited much significant[p
This study further evaluated the anti-hyperglycemic effects of methanol, ethyl acetate, and n-hexane crude extracts and ethyl acetate bulked fractions of Psidium guajava Linn leaves in alloxan-induced diabetic mice and the results showed a significant anti-hyperglyceamic effect in reduction of high blood glucose levels in test groups treated with a dose of 100mg/kg of each crude leaf extract of P. guajava as compared with negative and positive controls
Summary
Diabetes mellitus elucidates a metabolic disease characterized by abnormal hyperglycemia which alters the metabolism of lipids, carbohydrates, proteins arising from insulin-deficiency or insensitivity of target cell to insulin secreted in the body [37]. Cases of type 2 diabetes mellitus (T2DM) have been increasing in contrast to cases of type 1 (T1DM), an autoimmune disease that often occurs due to the destruction of insulin-producing beta cells of the pancreas, and results to deficiency in insulin secretion[62][108]. Obesity is associated with T2DM and hyperlipidemia and hypertension[118]120]. The coexistence of these diseases is well known as metabolic syndrome, a highrisk factor for cardiovascular disease[104][114]. It is known that obesity results from disequilibrium between energy intake and expenditure[117], and obesity is known to be a strong risk factor for Type diabetes associated with insulin resistance[14][113]
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