Laboratory evaluation of Indian medicinal plants as repellents against malaria, dengue, and filariasis vector mosquitoes.

Abstract

Mosquito-borne diseases have an economic impact, including loss in commercial and labor outputs, particularly in countries with tropical and subtropical climates; however, no part of the world is free from vector-borne diseases. Mosquitoes are the carriers of severe and well-known illnesses such as malaria, arboviral encephalitis, dengue fever, chikungunya fever, West Nile virus, and yellow fever. These diseases produce significant morbidity and mortality in humans and livestock around the world. In view of the recently increased interest in developing plant origin insecticides as an alternative to chemical insecticides, in the present study, the repellent activity of crude hexane, ethyl acetate, benzene, chloroform, and methanol extracts of leaf of Erythrina indica and root of Asparagus racemosus were assayed for their repellency against three important vector mosquitoes, viz., Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus. The crude extract was applied on a membrane used for membrane feeding of unfed mosquitoes in a 1-ft cage. About 50 unfed 3-4-day-old laboratory-reared pathogen-free strains of A. stephensi, A. aegypti, and C. quinquefasciatus were introduced in a 1-ft cage fitted with a membrane with blood for feeding with temperature maintained at 37 °C through circulating water bath maintained at 40-45 °C. Three concentrations (1.0, 2.0, and 5.0 mg/cm(2)) of the crude extracts were evaluated. Repellents in E. indica afforded longer protection time against A. stephensi, A. aegypti, and C. quinquefasciatus than those in A. racemosus at 5.0 mg/cm(2) concentration, and the mean complete protection time ranged from 120 to 210 min with the different extracts tested. In this observation, these two plant crude extracts gave protection against mosquito bites; also, the repellent activity is dependent on the strength of the plant extracts. These results suggest that the leaf extract of E. indica and root extract of A. racemosus have the potential to be used as an ideal eco-friendly approach for the control of mosquitoes. This is the first report on the mosquito repellent activity of the reported A. racemosus and E. indica plants.