EVALUATION OF ANTIOXIDANT POTENTIAL OF GREEN SYNTHESIZED TABEBUIA PALLIDA SILVER NANOPARTICLES

Abstract

Plant mediated green synthesis of silver nanoparticles has gained much importance in the current scenario. In this study, Tabebuia pallida leaves were used as a reducing agent for the sunlight mediated green synthesis of silver nanoparticles. The formation of silver nanoparticles was confirmed by UV-Vis spectroscopy which exhibited absorption maxima at 450 nm which is the characteristic of silver nanoparticles. Further, the characterization of the silver nanoparticles were carried out using Fourier transform infrared spectroscopy analysis, Field Emission Scanning Electron Microscope, Dynamic Light Scattering, and X-ray diffraction analysis. The size of the synthesized silver nanoparticles was found to be ranging between 31.76 nm-50.36 nm and is spherical in shape. The crystalline nature of the synthesized nanoparticles was confirmed by x-ray diffraction. Furthermore, the green synthesized silver nanoparticles were evaluated for their free radical scavenging activity using DPPH Radical Scavenging assay, ABTS radical scavenging assay, Hydroxyl radical scavenging activity, Reducing Power assay and Nitric oxide radical scavenging assay compared with the standard antioxidant Quercetin. Green synthesized silver nanoparticles exhibited a higher antioxidant activity against all the tested free radicals. From this evidences, it could be concluded that the green synthesized Tabebuia pallida silver nanoparticles can be used as a potential antioxidant for pharmaceutical applications.