In vitro antioxidant and anticancer activity of methanolic extract of Alangium salvifolium subsp. hexapetalum (Wangerin)

Abstract

The present study was implemented for revealing the antioxidant and anticancer effect of the aerial parts of methanolic extract of Alangium salvifolium subspecie hexapetalum (Wangerin). Preliminary screening of the methanolic extract was carried out to determine the presence of plant secondary metabolites such as alkaloids, glycosides, flavonoids, terpenoids, steroids, and sterols. Free radical scavenging activity was evaluated using DPPH radical scavenging assay, ABTS radical scavenging assay and reducing power assay. Invert phase contrast microscope and MTT assay method was used to analyse the number of viable cells using HT29 cell lines treated with different concentrations of the aerial parts of methanolic extract of Alangium salvifolium subspecie hexapetalum (Wangerin). The preliminary phytochemical analysis of the methanolic extract of Alangium salvifolium subspecie hexapetalum (Wangerin) unveil the presence of alkaloids, glycosides, flavonoids, tannins, phenolic compounds, carbohydrates and proteins as secondary metabolites. The free radical scavenging activity also revealed the presence of good antioxidant activity in the extract. A dose dependent growth inhibition of HT29 cell lines were seen when treated with methanolic extract of aerial parts of Alangium salvifolium subspecie hexapetalum (Wangerin). As the concentration of extract increases the percentage of viable cells decreases. The IC50 value for radical scavenging activity of methanolic extract was found to be 175μg/ml (DPPH radical scavenging assay) and 40μg/ml (ABTS scavenging assay). The IC50 value for anticancer activity was found to be 30.22μg/ml. The investigational outcome proves that the methanolic extract of aerial parts of Alangium salvifolium subspecie hexapetalum (Wangerin) showed promising antioxidant and cytotoxic activities.