Biochemical Screening, Fabrication of Green Nanoparticles and Its Antimicrobial, and Antioxidant Studies of Endophytic Fungus Phlebia Species.

Abstract

Endophytes are organism dwelling totally dynamic and novel biotopes this makes them able to produce novel biochemicals that may become assets to the future. This study aims at understanding the biochemical components of the endophytic fungus Phlebia sp. synthesis of gold and silver nanoparticles from it, and the antimicrobial as well as antioxidant ability of these green synthesised nanoparticles. Aqueous fungal extract was subjected for HRLCMS analysis which revealed 34 biochemicals within the extract. Silver and gold nanoparticles were also produced from the fungal extract. UV-vis analysis revealed a peak at 450nm for silver nanoparticle and 550nm for gold nanoparticles. FESEM analysis confirmed the presence of these nanoparticles with its spherical shape. Both of these nanoparticles were able to produce a conspicuous zone of inhibition in the antimicrobial tests against Escherichia coli, Salmonella paratyphi. For both of the organisms under study, a concentration-dependent expansion of the zone of inhibition was discovered in the nanoparticles. However, with silver nanoparticles, a relatively high zone of inhibition and vulnerability of the organism was discovered. Four in vitro free radical scavenging assays, including the DPPH, Hydroxyl, Superoxide, and Nitric oxide radical scavenging assays, were used for antioxidant analysis. The results of every test demonstrated that green synthesisedsilver nanoparticles had higher activity than gold nanoparticles. All of the tests showed that silver nanoparticles were more active than gold nanoparticles with the maximum value of 86.254 ± 0.296% being discovered at the greatest concentration of superoxide radical scavenging assay.