Evaluation of Antibacterial Activity of Iron Oxide Nanoparticles Against Escherichia coli

Mehrdad Khatami,Marcos Augustino Lima Nobre,Mansour Khatami,Farideh Mosazade,Hakim Azizi,Farzad Minab Poodineh,Hadi Mirzaeei,Mohammad Reza Aflatoonian,Sam Khraazi,Ahmad Hooshmand

doi:10.15171/ijbsm.2017.31

Abstract

Introduction: Considering the usefulness of metal oxide nanoparticles in biology and biomedicine, iron oxide nanoparticles were biosynthesized using bioresource engineering to evaluate its antibacterial activity against Escherichia coli. Methods: Macrodilution method was used for calculating the lowest concentration which prevented the growth of bacteria (minimum inhibitory concentration [MIC]), and the lowest concentration that destroyed all bacterial cells (minimum bactericidal concentration [MBC]). Results: The lowest concentration of iron oxide nanoparticles that inhibited the growth of E. coli (MIC) was recorded at 250 µg/mL. On the other hand, the MBC of iron oxide nanoparticles was calculated at 500 µg/mL. Conclusion: Iron oxide nanoparticles were produced by a green and eco-friendly, simple and inexpensive method. The results showed the inhibitory effect of iron oxide nanoparticles on E. coli at 250 µg/mL. This may suggest using these nanoparticles as potential antibacterial agents.

Highlights

Introduction: Considering the usefulness of metal oxide nanoparticles in biology and biomedicine, iron oxide nanoparticles were biosynthesized using bioresource engineering to evaluate its antibacterial activity against Escherichia coli
Transmission electron microscopy (TEM) images of biosynthesized iron oxide nanoparticles are shown in Figure 1; the particle size range was from 1 to 12 nm
The antibacterial activity of iron oxide nanoparticles was investigated at concentrations of 2000, 1000, 500, 250 and 125 μg/mL

Summary

Introduction

Considering the usefulness of metal oxide nanoparticles in biology and biomedicine, iron oxide nanoparticles were biosynthesized using bioresource engineering to evaluate its antibacterial activity against Escherichia coli. Antimicrobial agents considerably help to prevent and treat infectious diseases in humans and animals; the increase of resistant microbial strains has turned to a serious challenge in medicine.[1,2,3,4] Nanomaterials have attracted the researchers’ interest due to their significant antibacterial activity.[5,6] In recent years, antimicrobial nanoparticles have paved the way for medicine and biotechnology.[7,8] Nanoparticles with high antimicrobial activity are a new class of biomedical materials.[9,10,11,12,13] Iron oxide nanoparticles have a high specific surface area, so they are able to interact with bacterial surface structures. We aimed to evaluate the antibacterial impacts of iron oxide nanoparticles on a common bacterial strain in clinical practice.[18,19]

Objectives

Methods

Results

Conclusion