Evaluating Homocysteine, By HPLC, HBA1C And Fasting Blood Glucose Levels In Patients With Type 2 Diabetes Mellitus

Abstract

Background: The objective of the study was to evaluate the levels of Homocysteine, HBA1C and fasting plasma glucose in serum of type 2 diabetic patients as a parameter and correlation coefficient between Homocysteine and each of HBA1Cand fasting blood glucose (FPG). Materials and Methods: The present study was applied on 30of type 2 diabetic patients and 30 healthy controls of same age and gender. Fasting blood samples were collected from each patient and control and assessed by measurement of serum Homocysteine (HPLC assay), FPG (Glucose Oxidation Peroxidase method) and HbA1c (Immunoturbidimetric method) levels. Result and discussion: : In this project, the mean value of serum Homocysteine in diabetic patients was 26.5 ± 1.73 μmol/L. It was increased significantly compared to controls (0.98 ± 0.11 μmol/L, p=0.000). The mean value of FBG in diabetic patients was 230.3 ± 6.73 μmol/L. Also, it was increased significantly compared to controls (89.5 ± 4.7 mg/dL, p=0.000). Similarly, the mean value of HbA1c in diabetic patients was 11.5 ± 0.99 μmol/L. It was increased significantly compared to controls (5.1 ± 0.13 mg/dL, p=0.000). Our findings demonstrated significant positive correlation between serum Homocysteine and fasting blood glucose levels (r=0.811,p=0.001), and also between serum Homocysteine and HbA1c levels (r=0.324, p=0.02).