Ethanol Extract of Sesamum indicum Linn. Inhibits FcεRI-Mediated Allergic Reaction via Regulation of Lyn/Syk and Fyn Signaling Pathways in Rat Basophilic Leukemic RBL-2H3 Mast Cells.

Hyun Ju Do,Kwang-Il Park,Tae Woo Oh

doi:10.1155/2019/5914396

Hyun Ju Do, Kwang-Il Park + Show 1 more

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https://doi.org/10.1155/2019/5914396

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Abstract

This study is aimed at determining whether Sesamum indicum Linn. beneficially influences FcεRI-mediated allergic reactions in RBL-2H3 mast cells; it is also aimed at further investigating Lyn/Fyn and Syk signaling pathways. To examine the antiallergic effect of Sesamum indicum Linn. extract (SIE), we treated antigen/immunoglobulin E- (IgE-) sensitized mast cells with extracts of various concentrations. We examined the degranulation release and concentrations of inflammatory mediators. Additionally, the expressions of genes involved in the FcεRI and arachidonate signaling pathways were examined. SIE inhibited the degranulation and secretion of inflammatory mediators in antigen/IgE-sensitized mast cells. SIE reduced the expressions of FcεRI signaling-related genes, such as Syk, Lyn, and Fyn, and the phosphorylation of extracellular signal-regulated kinase in antigen/IgE-sensitized mast cells. Additionally, in late allergic responses, SIE reduced PGD2 release and COX-2 and cPLA2 phosphorylation expression in FcεRI-mediated mast cell activation. Lastly, 250–500 mg/kg SIE significantly attenuated the Ag/IgE-induced passive cutaneous anaphylaxis (PCA) reaction in mice. The potent effect of SIE on RBL-2H3 mast cell activation indicates that the extract could potentially be used as a novel inhibitor against allergic reactions.

Highlights

Mast cells are widely distributed across the human body and are present in various regions, including the skin, respiratory, and digestive systems [1]
The present study investigated the antiallergic activities of the Sesamum indicum Linn. extract (SIE) against FcεRImediated allergic reactions in mast cells and the mechanism by which SIE suppresses these allergic reactions
To determine the cell viability rate of SIE treatment on immunoglobulin E- (IgE-)induced RBL-2H3 mast cells, we performed the MTT assay and found that SIE did not adversely affect cell viability at concentrations of 100, 300, and 500 μg/mL (Figure 1(a)). β-Hexosaminidase release as a marker of degranulation in mast cells is used as a good indicator of the degree of allergic reaction [11]

Summary

Introduction

Mast cells are widely distributed across the human body and are present in various regions, including the skin, respiratory, and digestive systems [1]. These cells generate a large number of mediators that induce inflammation and immune responses to various external stimuli [1, 2]. They are crucial in natural and adaptive immune responses. We have used RBL-2H3 cells as a model cell line to assess the inhibitory effect of Lyn/Syk and Fyn against FcεRI-mediated mast cell activation

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