Estimation of the relative DNA content in species of the genus Spiraea, sections Chamaedryon and Glomerati by flow cytometry

V A Kostikova,A S Ernst,T N Veklich,E Yu Mitrenina,E V Shabanova(Kobozeva),M S Voronkova,A A Kuznetsov

doi:10.15421/2019_71

Abstract

The relative DNA content was studied in seven species of the genus Spiraea L., section Chamaedryon Ser., and in two species, section Glomerati Nakai, from 28 natural populations growing in Asian Russia. The cell nuclei were isolated from a leaf tissue. The relative intensity of fluorescence was measured using flow cytometry of propidium iodide-stained nuclei. The analysis was performed using a CyFlowSpace device (Germany, Sysmex Partec) with a laser radiation source of 532 nm. Fresh leaves of Solanum lycopersicum cv. ‘Stupice’ were used as an internal standard. Data on the relative DNA content are presented for the first time for S. flexuosa Fisch ex Cambess. (0.42–0.47 pg), S. ussuriensis Pojark. (0.49–0.52; 0.85 pg), S. alpina-Pall. (0.49–0.51 pg), S. media Schmidt. (0.45; 0.98–1.01 pg), S. trilobata L. (0.46 pg), S. hypericifolia L. (0.49–0.52 pg) and S. aquilegifolia Pall. (0.48–0.51 pg). Mesophytic species of the genus Spiraea growing in the forest zone (S. chamaedryfolia L. and S. media) exhibit a 2-fold higher relative DNA content (C-value) compared to xerophytic species (S. hypericifolia, S. crenata L., S. aquilegifolia and S. trilobata) and species confined only to mountain areas (S. alpinа, S. trilobata and S. aquilegifolia).

Highlights

Studies on changes in the genome size of one species or closely related species of plants from different areas allow us to consider the evolutionary processes and their types as well as to predict steps of evolution (Marda et al 2008)
Calospira, growing in Asian Russia is determined for the first time by flow cytometry according to the fluorescence relative intensity (Table 1)
Glomerati Nakai, from 28 natural populations growing in Asian Russia

Summary

Introduction

Studies on changes in the genome size of one species or closely related species of plants from different areas allow us to consider the evolutionary processes and their types as well as to predict steps of evolution (Marda et al 2008). The section Glomerati includes species with a sessile umbel and is isolated from the section Chamaedryon Representatives of these sections are common in Eurasia; they are not found in North America (Slavkina, 1972). In Asian Russia, species of the section Chamaedryon – S. alpina Pall., S. chamaedryfolia L., S. media Schmidt., S. pubescens Turcz., S. trilobata L., S. crenata L., and Estimation of the relative DNA content species of the section Glomerati – S. hypericifolia L. and S. aquilegifolia Pall should probably be considered the fully formed plants of the genus Spiraea. The relative DNA content of representatives of the genus Spiraea, sections Chamaedryon and Glomerati, growing in Asian Russia has not been previously studied. The aim of the study was to compare the relative DNA content in species of the genus Spiraea, sections Chamaedryon and Glomerati

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