Abstract

Paan (Piper betle L., family Piperaceae) is an important evergreen cultivated crop of India. We estimated genetic diversity by using morphological and RAPD markers in four cultivars, namely Piper betle L. var. Bali, var. Chandrakanta, var. Jhanji and var. Kala Bangla. We had used morphological markers like leaf length, leaf width, and petiole size. By using the morphological markers, hierarchical cluster analysis was carried out, which grouped these four cultivars into two major clusters. In molecular marker analysis, a total of ten RAPD primers used, generating 43 number of amplified bands. Among them, 15 number of polymorphic bands and seven unique bands were found. The genetic diversity and relatedness among the four cultivars were computed using Jaccard’s similarity coefficient. The dendrogram grouped all the four cultivars into two main clusters. This RAPD banding patterns can be useful for genetic diversity studies, for cultivar selection, and to marker assist breeding programs.

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