Esterase isozymes are useful to track introgression between Allium fistulosum L. and A. cepa L.

Abstract

Polyacrylamide gel electrophoresis (PAGE) was used to study the polymorphism of esterases in Allium cepa L. and A. fistulosum L. Two varieties of each species, an A. fistulosum × A. cepa interspecific F1 hybrid, and (A. fistulosum × A. cepa) hybrid derivatives were analyzed for determination of banding patterns upon staining with α- and β-napthyl acetate substrates of esterase. Complex band patterns were observed. In total, 10 bands were detected between A. cepa and A. fistulosum — five inA. cepa, six in A. fistulosum with only one band common to both species. With the exception of one band unique to A. fistulosum which appeared only when stained with α-substrate, extracts of both A. cepa and A. fistulosum leaf tissue exhibited the same bands when stained with both α- and β-substrates. Bands stained with the different systems are distinguished by color: α-substrate always appeared black, while bands stained to β-substrate are always red. Esterase bands were assigned into 5 presumptive loci of four zones of activity with according to the migration distance of the bands from the front, color of each band upon staining with α- and β-substrates, and segregation observed in crosses and hybrid derivatives. Esterase enzymes detected in this study appear to be monomeric. Polymorphisms were identified between A. cepa and A. fistulosum by esterase banding patterns. Esterase enzymes provide an additional marker in monitoring introgression of foreign germplasm in interspecific onion breeding.