Örtüaltından toplanan Trialeurodes vaporariorum (Westw.) ve Bemisia tabaci (Genn.) (Hemiptera:Aleyrodidae) türlerinin poliakrilamid jel elektroforez yöntemiyle belirlenmesi

Abstract

The whiteflies are one of the major pests of field and greenhouse crops throughout the world. They cause damage both directly by feeding and indirectly through the excretion of honeydew and as a vector of plant virus diseases. Regular monitoring of whiteflies, by using polyacrylamide gel electrophoresis (PAGE), can indicate movement/migration between geographical areas, indicate both inter and intra-strain variation/polymorphism, evaluate potential contamination between laboratory strains and also disclose esterase profiles for individual insects. Electrophoresis is one of the main technique for separating proteins and nucleic acids for a variety of purposes in most of the laboratories today. Densities of the esterase bands obtained from the PAGE have an important role in the possible determination of the insecticide resistance levels in some pests. At the same time, esterase banding patterns on electrophoretic gels have been used to provide an extremely reliable method for determining biotypes in especially whiteflies. There are many different biotypes in Bemisia tabaci . Both B. tabaci biotypes and Trialeurodes vaporariorum , which is a different species, are easily identified from each other with this method. In this study, 18 different whitefly populations collected from Aegean and Mediterranean Regions of Turkey were analyzed with PAGE. The standard populations from England were also used as well as the collected populations. According to the obtained results, the 11 populations were determined as T. vaporariorum , while 7 were B. tabaci . The typical B-biotype band (E0.14) is found in all B. tabaci populations.