Abstract
An efficient procedure for identification of tulip cultivars in dry bulb stage, based on polymorphism in the isozymes of esterase, is described. Out of 91 cultivars (excl. mutants) which were analyzed for their esterase banding patterns, 78 (86%) could be uniquely identified using native PAGE of bulb protein extracts. A group of 14 Darwin hybrids showed strongly resembling banding patterns; they all originate from the same breeder and most likely have the same parents. Mutants could not be discriminated. The esterase patterns were not influenced by type of bulb scale used for extraction (inner vs outer scale), bulb size (mature bulb or clister) and bulb origin (different lots, grown on sand or clay). However, bulb storage did affect the esterase pattern in several cultivars; generally, some bands appeared or became more intense in the lowest region of the banding pattern after storage of the bulbs.
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