Epidemiological and molecular characterization of antigens extracted from Hydatid cysts of camel, cattle and donkeys in Egypt

Abstract

Cystic echinococcosis or Hydatid disease is recognized as an important worldwide distributed disease from the clinical, economical and zoonotic point of view. In the present work, 180 camels and 90 cattle freshly slaughtered at Cairo abattoir and 120 donkeys scarified at Giza zoo, were inspected for infection by Hydatid cysts (HC) in Egypt. The highest incidence of HC infection was 18.9% in Camel, 14.2% in donkeys and the lowest 3.3% in cattle. Regarding the site of HC infection was 94.3% and 90.2% in Camel lungs and donkeys liver. The fertility of HC was 79.24% and 29.4% from camel and donkeys, while, all inspected hydatid cysts collected from cattle were found calcified. Germinal membranes of fertile HC were used for DNA extraction followed by PCR amplification. It was used for identification of internal transcribed spacer gene1(ITS1)from camel and donkeys by using specific primer. The amplified DNA fragment was further analyzed by PCR mediated restriction fragment length polymorphism (PCR RFLP) using two restriction enzymes (MSP1 and RSA1). The PCR yielded similar amplified DNA band of the same molecular size marker at 1115 bp in different isolates of Hydatid. No band variation of ITS 1 gene could be detected by PCR- RFLP by using two restriction enzymes. Amplification product of ITSI after digestion with MSP1 showed at 661 bp, while those restricted with RSA1 enzyme appeared at 745 bp. Keywords: Molecular, Hydatid cyst, Antigens, PCR, Camel and Donkeys, Egypt.