Enzymatic inhibition of lysine transport across rat and turtle small intestine

Abstract

1. 1. Trypsin, Phospholipase- d and Neuraminidase at different concentrations inhibited significantly ( P < 0.001) lysine accumulation in rat intestinal cells, whereas Chymotrypsin did not show any significant effect. 2. 2. Trypsin, Chymotrypsin and Phospholipase- d did not show any significant change in lysine accumulation when incubated with mucosal strips isolated from turtle intestine; however, Neuraminidase caused a significant decrease in lysine uptake by these strips. 3. 3. Unidirectional lysine influx across the rat small intestine was significantly reduced ( P < 0.01) by the presence of Trypsin, Phospholipase- d and Neuraminidase in the preincubation solution, and its influx across the turtle small intestine was significantly inhibited ( P <0.01) by Neuraminidase only. 4. 4. The cell water content and the cell volume were not altered by preincubating the intestinal tissues with any of the enzymes in both animals.