Enzymatic Determinations of Glucose

Abstract

Publisher Summary This chapter highlights that glucose is important in the metabolism of most cells—animal, plant, and microbe—and a number of enzymes have been identified which catalyze the reactions of glucose. It discusses that enzymatic methods have a number of theoretical advantages, and therefore, it is quite logical that much attention has been devoted to the subject of enzymatic determinations of glucose. In all instances, the theoretical advantages do not necessarily apply in the defined procedures. Early qualitative and quantitative tests in which yeast was employed for either the identification or measurement of glucose might be considered to have an enzymatic basis as the desired actions were accomplished by means of the enzymatic processes in living yeast cells. This chapter describes the history and properties of glucose oxidase. It presents an account of the utilization of glucose oxidase for the measurement of glucose in urine and blood, and for other test situations that may be of interest in clinical chemistry.