Abstract

To treat diseases brought on by drug-resistant microbes, researchers are presently using nanotechnology as one of the tools for studying the most obscure corners of medical sciences. The biological synthesis regarding Ag/TiO2 synergism in prodigiosin pigment was the primary focus of the presented work. Inhibitory and bactericidal properties of silver nanoparticles (AgNPs) have long been recognized. Titanium dioxide nanoparticles (TiO2 NPs) are employed as antibacterial agents in a variety of therapeutic sources. With using Nd:Yag laser (1064 nm), the composition has been synthesized by using environmentally friendly laser ablation in a liquid method. UV–visible spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM) have been utilized for the analysis of the synthesized NPs. Optimal biosynthesis results showed a rise in the intensity of Surface Plasmon Resonance (SPR) bands of the NPs with shifting at 300 nm. TiO2 absorption peak at 230 nm. The results showed that the UV visible absorbance regarding prodigiosin pigment was at OD529. AgNPs/TiO2 exerted maximum antibacterial activity towards (Streptococcus, Bacillus subtilis, E. coli, Proteus vulgaris) Gram-negative and Gram-positive bacteria. The various Gram-negative and Gram-positive bacteria were subjected to various treatments (prodigiosin with Ag, prodigiosin pigment, and prodigiosin synergism with Ag/TiO2). The synergism is the best for antimicrobials than each one alone.

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