Abstract
The present study was aimed to biosynthesis of silver nanoparticles by using rhaminolipid produced from local isolate Pseudomonas aeruginosa as reducing and stabilizing agent. Silver nanoparticles (AgNPs) synthesized by green method have shown several applications such as biomedical, anticancer, bio sensing, catalysis etc. Characterization study of purified bioemulsifier using thin layer chromatography (TLC) was demonstrated that the biosurfactant contains mono, and di- rhamnolipid with Rf values of 0.86 and 0.36 respectively. Optimization results of biosynthesis silver nanoparticles were revealed that an increasing in intensity of Surface Plasmon Resonance (SPR) bands of nanoparticles with shifting at wavelength (400 nm). Also optimum synthesis of AgNPs was at pH 5, Temperature 40℃, reaction time 5 minutes with concentration of rhaminolipid as reducing agents (2×10-3w/v) and Silver ion concentration (6×10-3 mol/L). The result of X-ray diffraction was indicated that the size of silver nanoparticles observed was 38 nm and show relatively stable peak at -23.2 mV. Finally, the minimum inhibitory concentration of Ag NPs against human pathogenic bacteria obtained at concentration (1mg/ml) for both gram negative and gram-positive bacteria. The results of anti-inflammatory effects of Ag NPs obviously, cleared that the infection of test animals treated with AgNPs were completely healed after 6 days of treatment, while the animals treated with fucidin (as control) not exhibited any healing in the infection.
Highlights
Metal nanoparticles have a key role in many applications as they show superior physicochemical properties due to their significantly small size and very high surface area, This case distinguishes nanoscale and opens its horizons in many application, where it can penetrate and damage the walls of microorganisms
Identification of the biosurfactant producing isolate In the current study the selected bacterial isolate for biosurfactant production was identified by using VITEK2 compact system based on biochemical testes
The selected isolate for biosurfactant production was in 97% similarity with Pseudomonas aeruginosa
Summary
Metal nanoparticles have a key role in many applications as they show superior physicochemical properties (optical, catalytic activity, magnetic, Electronic, and antibacterial properties) due to their significantly small size and very high surface area, This case distinguishes nanoscale and opens its horizons in many application, where it can penetrate and damage the walls of microorganisms. The chemical reduction method for synthesis silver nanoparticles is still expensive and employ hazardous organic solvents and toxic reducing agents [14]. Recognizing the importance of developing eco-friendly methods for the synthesis of biologically active nanoparticles, scientists have recently started looking into research relating to the synthesis of metallic nanoparticles with the additional use of biosurfactant as capping agents [17]. As well as the potential application of the synthesized nanoparticles in vitro as antibacterial activity against human pathogenic bacteria was studied and in vivo as anti-inflammatory activity of AgNPs MATERIALS AND METHODS Bacterial isolate and culture media The bacterial isolate used in the present study was previously isolated from petroleumcontaminated soil and exhibited high biosurfactant produced and obtained from biotechnology department. The medium left to cool and inoculated 1% of the selected bacteria isolate (1×108 CFU, OD=0.5) and incubated in a shaker incubator at 30°C at 120 rpm for 96h
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