Abstract
Background: Lamotrigine, an anticonvulsant drug, requires sensitive detection methods in biological and pharmaceutical matrices. Current methods struggle with interference and sensitivity, necessitating the development of improved analytical techniques. Objective: This study aims to develop and validate a highly sensitive HPLC method enhanced by salicylaldehyde derivatization for detecting lamotrigine in pharmaceutical and biological samples, and to differentiate it from Piracetam when co-administered. Methods: A reverse-phase HPLC method was employed, utilizing salicylaldehyde as a derivatizing agent to induce a bathochromic shift in lamotrigine from 315 nm to 415 nm. Optimization of the derivatization reaction included adjustments of pH, reagent concentration, temperature, and time. The standard addition method was applied to evaluate the recovery rates from spiked samples. Results: The optimized method demonstrated a linear calibration range of 1-5 µg/ml with a determination coefficient (R²) of 0.998. Recovery of lamotrigine from pharmaceutical preparations averaged 96.5%, while recovery from deprotonated serum and urine samples of healthy volunteers was 96% with a relative standard deviation (RSD) of 6%. Conclusion: The developed method offers a robust and highly sensitive approach to detect and distinguish lamotrigine in the presence of Piracetam, proving effective for both pharmaceutical and biological sample analysis.
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